POP7 antibody

Catalog No. ABIN1108698

Product Details anti-POP7 Antibody

Target: POP7 (Processing of Precursor 7, Ribonuclease P/MRP Subunit (POP7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POP7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.

Clone: 1F11

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Dissolve in 200 μL buffer for a 0.5 mg/mL antibody concentration in 1 % BSA

Concentration: 0.5 mg/mL (when dissolved at 0.5 mg/mL, the BSA concentration will be 1%)

Buffer: 10 mM Ammonium Bicarbonate buffer, 2 mg BSA

Precaution of Use: Be careful opening the vial since the antibody resides in a Vacuum.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C or Add up to 0.05% Sodium Azide for long term storage.

Target Details for POP7

Target: POP7 (Processing of Precursor 7, Ribonuclease P/MRP Subunit (POP7))

Alternative Name: POP7 (POP7 Products)

Synonyms: 0610037N12Rik antibody, AI852017 antibody, Rpp20 antibody, RPP2 antibody, RPP20 antibody, POP7 homolog, ribonuclease P/MRP subunit antibody, processing of precursor 7, ribonuclease P family, (S. cerevisiae) antibody, Pop7 antibody, POP7 antibody

Background: The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.Synonyms: RPP20, Ribonuclease P protein subunit p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7

Molecular Weight: 20 kDa

Gene ID: 10248

NCBI Accession: NP_005828

UniProt: O75817