E. Coli antibody

Catalog No. ABIN110776

Product Details anti-E. Coli Antibody

Target: E. Coli

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: This E. Coli antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Affinity Chromatography on Protein A

Immunogen: Escherichia coli J5 cells.

Clone: 2D7-1

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence. ELISA: (a 1 mg/mL solution has been used at dilutions of up to 1/1500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for E. Coli

Target: E. Coli

Abstract: E. Coli Products

Target Type: Bacteria

Background: Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).Synonyms: EHEC, EPEC, ETEC, Escherichia coli