Histone H2A antibody

Catalog No. ABIN1107544

Product Details anti-Histone H2A Antibody

Target: Histone H2A

Reactivity: Human, Rat, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat, hamster

Purification: Protein A agarose

Immunogen: Protein fraction extracted from the mitotic chromosome

Clone: C10037

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Histone H2A

Target: Histone H2A

Abstract: Histone H2A Products

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multIPPle different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. The role of histone variants, and specially those of H3 and H2A, in various nuclear processes has been long appreciated. There are at least three different families of H2A variants present in a variety of organisms from yeast to mammals, and the degree of conservation among members of each family is greater than that of the canonical H2A. H2AX is thought to play a role in DNA double-strand break repair, the serine in the SQEY motif of H2AX is phosphorylated at the site of the DNA damage and serves as a signal for the recruitment of repair proteins. Macro H2A1, another H2A variant, has been shown to have a role in X-chromosome inactivation and dosage compensation in mammals, where it is found to localize to the inactive X after silencing has been established.Synonyms: H2A Histone Family, HIST1H2A

Molecular Weight: 14 kDa