CD43 antibody

Catalog No. ABIN1106370

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with CD43 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A agarose

Immunogen: PHA-activated human T cell line maintained with IL-2 containing media

Clone: 1D4

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water. After reconstitution, the IgG concentration should be 1 mg/mL.

Buffer: PBS ( pH 7.2) contraining no preservatives, 1 % sucrose

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 / Leukosialin (SPN Products)

Background: CD43 antigen is a heavily sialylated single chain membrane glycoprotein with both molecular weight and antigenicity depending on the nature and complexity of the attached O-linked polysaccharides. CD43 is an integral membrane protein with extracellular domain (235 aa), transmembrane domain (23 aa) and intracytoplasmic domain (123 aa). The extracellular domain of CD43 has been shown to react with many ligand such as ICAM-1 (CD54), E-selectin, galectin-1, MHC-I and human serum albumin (HSA). The intracytoplasmic domain, which is highly conserved between species, is constitutively phosphorylated on serine residues. The level of phosphorylation increases after cell activation. This cell surface glycoprotein is also called leukosialin, sialophorin or GPL115. A soluble form of CD43 is also present in human serum. Thymocytes, CD4+ T lymphocytes and monocytes express more of a 115 kDa CD43 isoform, whereas a 130 kDa isoform is found mostly on activated CD4+ T cells, CD8+ resting and activated T cells, neutrophils, platelets and B cells. CD43 plays a role in the regulation of lymphoid cell adhesion, cellular maturation and activation although the nature of this participation remains controversial. For example, the structural characteristics of the CD43 extracellular domain indicate that this molecule has both adhesive and anti-adhesive properties. O-glycosylated chains of CD43 such as a sialyl Lewis X (sLex) epitope, a ligand for both P- and E-selectin, potentiate binding to lectin-like molecules. However, the adhesion molecule function for CD43 is counteracted by the presence of negatively charged sialic acid residues on its extracellular domain. In addition to the strong structural evidence for the involvement of CD43 in cell interactions, experimental data suggest contradictive role for CD43 in regulating cell contacts.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gene ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity