CD19 antibody

Catalog No. ABIN1105967

Product Details anti-CD19 Antibody

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone HD37 is specific for CD19, it recognizes a 95 kDa transmembrane glycoprotein. Testing by flow cytometry using a 'lyse-wash' method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count: Product code: AM39014AC-N (anti-CD19 APC) n: 9 Mean % positive: 9,16 S.D.: 1,95 % CV: 21,31 ------------------------------------------ Product code: AM39014FC-N (anti-CD19 FITC) n: 9 Mean % positive: 10,80 S.D.: 2,14 % CV: 19,79 ------------------------------------------ Product code: AM39014RP-N (anti-CD19 PE) n: 9 Mean % positive: 10,57 S.D.: 2,06 % CV: 19,49 ------------------------------------------

Purification: Affinity chromatography

Clone: HD37

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Synonyms: B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background: Progenitor B cells mature in bone marrow and subsequently appear as mature CD19+ B cells in blood. After contact with foreign antigens and the appropriate T cell help, they may further differentiate to specific antibody-producing plasma cells.The function of the CD19 Molecule is related to signal transfer and is involved in regulation of B cell proliferation. CD19 is considered to be a characteristic B cell marker and therefore commonly used in routine immunophenotyping. Detection of CD19 expressing cells is important in the diagnosis of leukemic precursor B cells (pre-B ALL), mature B cells (B ALL), and plasma cells. Distinction between subtypes of these (acute) leukemias can be performed using CD19 antibodies together with monoclonal antibodies to cytoplasmic or membrane Ig. A large number of B cell disorders can be effectively characterized by expression of CD19 and one or more additional antigens. One example is hairy cell leukemia (HCL), which shows specific expression of CD11c, CD19, CD20 and CD103. The combination CD103/CD19 is an important tool for diagnosis of HCL. Other valuable antibody combinations for distinction between different leukemias using CD19 antibodies are CD5/CD19 (e.g. Chronic Lymphatic Leukemia), CD10/CD19 (common ALL and pre-B ALL) and CD19/cyCD79a (Acute B cell leukemia). CD19 may also be expressed on follicular dendritic cells.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12

Gene ID: 930

NCBI Accession: NP_001171569

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway