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Actin antibody (C-Term, pan)

Reactivity: Human, Rat, Mouse, Chicken, Snake WB, IF, IHC (fro) Host: Mouse Monoclonal AC-40 unconjugated

Catalog No. ABIN1105234

Datasheet as PDF

Target See all Actin Antibodies

Actin
Binding Specificity
16

15

13

12

11

4

2

2

2

2

2

2

1

1

1

1

1

1

1
C-Term, pan
Reactivity
98

79

75

25

21

21

12

11

7

6

5

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Human, Rat, Mouse, Chicken, Snake
Host
83

41

3

1

1
Mouse
Compatible Secondaries
Clonality
75

54
Monoclonal
Conjugate
73

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This Actin antibody is un-conjugated
Application
100

36

30

29

28

28

24

19

16

16

10

3

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification

Goat anti-Mouse IgG Affinity Chromatography

Immunogen

Synthetic Actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

Clone

AC-40

Isotype

IgG2a

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anti-Actin (AA 165-260) antibody
Reactivity: Human, Rat, Mouse, Rabbit, Dog WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Human, Rat, Mouse, Chicken, Dog, Cow, Pig, Guinea Pig WB, ELISA, IHC (p), IF (cc), IF (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Actin (C-Term) antibody
Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Actin antibody
Reactivity: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 367-377) antibody
Reactivity: Human, Rat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 21-70) antibody
Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 189-218) antibody
Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin (AA 38-67), (N-Term) antibody
Reactivity: Human, Mouse WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated

anti-Actin (C-Term) antibody
Reactivity: Human IHC (p), IHC (fro) Host: Rabbit Monoclonal S12-I unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Reconstitution

Restore with 1.2 % Sodium Acetate or neutral PBS.

Concentration

0.1 mg/mL

Buffer

1.2 % Sodium Acetate containing 2 mg BSA and 0.01 mg Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody at -20 °C. After reconstituion store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Actin

Abstract

Actin Products

Background

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.