RIF1 antibody (AA 2432-2444)
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- Target See all RIF1 Antibodies
- RIF1 (RAP1 Interacting Factor Homolog (RIF1))
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Binding Specificity
- AA 2432-2444
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This RIF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human RIF1. This antibody is expected to recognize both reported isoforms (NP_060621.3, NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%) Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-RNLQSRWRSPSHEN from the C-terminus of human RIF1 (NP_060621.3, NP_001171134.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%), Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RIF1 Primary Antibody
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- Application Notes
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Approved: Peptide-ELISA (1:8000), WB (1 - 3 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 270kD band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kD according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- RIF1 (RAP1 Interacting Factor Homolog (RIF1))
- Alternative Name
- RIF1 (RIF1 Products)
- Synonyms
- 5730435J01Rik antibody, 6530403D07Rik antibody, AU016181 antibody, AW549474 antibody, D2Ertd145e antibody, RGD1562474 antibody, fc38c03 antibody, wu:fc38c03 antibody, si:dkey-89p3.2 antibody, replication timing regulatory factor 1 antibody, RAP1 interacting factor homolog (yeast) antibody, RIF1 antibody, Rif1 antibody, rif1 antibody
- Background
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Name/Gene ID: RIF1
Synonyms: RIF1 - Gene ID
- 55183
- NCBI Accession
- NP_060621, NP_001171134
- UniProt
- Q5UIP0
- Pathways
- Stem Cell Maintenance
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