PLP1 antibody (C-Term)

Catalog No. ABIN1103681

Product Details anti-PLP1 Antibody

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Binding Specificity: C-Term

Reactivity: Human, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

Purification: Purified

Immunogen: Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4 % PFA fixed cells for 5-10 minutes at RT. Use 0.1 % triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4 % PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2 % triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS buffer,  pH 7.2, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: PLP1 / Myelin PLP (PLP1 Products)

Background: Name/Gene ID: PLP1

Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20

Gene ID: 5354