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Myc Tag antibody (AA 410-419) (HRP)

WB, ELISA, IHC Host: Rabbit Polyclonal HRP

Catalog No. ABIN1103151

Datasheet as PDF

Target See all Myc Tag products

Myc Tag
Binding Specificity
21

21

12

8

6

3

2

1
AA 410-419

Reactivity

Please inquire
Host
75

49

6

5

5

5

1

1
Rabbit
Compatible Secondaries
Clonality
77

58

10
Polyclonal
Conjugate
67

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is conjugated to HRP
Application
110

53

42

36

25

23

20

18

15

11

11

6

6

5

4

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Rabbit Serum.

Purification

Affinity purified

Immunogen

This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein

Isotype

IgG
anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Host: Rabbit Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivity: Human FACS Host: Mouse Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Host: Mouse Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB Host: Goat Polyclonal unconjugated

Application Notes

Approved: ELISA, IHC (1:500 - 1:2000), WB (1:2000 - 1:5000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions

For Research Use only
Format

Liquid

Reconstitution

deionized water

Concentration

Lot specific

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate

Preservative

Gentamicin sulfate

Precaution of Use

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freeze thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

Myc Tag

Abstract

Myc Tag Products

Target Type

Tag