Interleukin 17a antibody

Catalog No. ABIN1102463

Product Details anti-Interleukin 17a Antibody

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human IL-17A

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-17A.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.9 - 1.3 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7397 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-17A (50 ng/mL), a concentration of 0.9-1.3 μg/mL of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A / IL17 (IL17A Products)

Synonyms: CTLA8 antibody, IL-17 antibody, IL-17A antibody, IL17 antibody, Ctla-8 antibody, Ctla8 antibody, Il17 antibody, ChIL-17 antibody, IL-17F antibody, IL17A antibody, CTLA-8 antibody, interleukin 17A antibody, IL17A antibody, Il17a antibody

Background: Name/Gene ID: IL17A
Family: Interleukin

Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17

Gene ID: 3605

UniProt: Q16552