6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Purification
Purified
Sterility
Sterile filtered
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
WB, ELISA, IP, DB, ISt
Host: Mouse
Monoclonal
HIS-H8
unconjugated
Application Notes
Approved: ELISA, WB (1:1000)
Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
avoid freeze thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.