Follistatin antibody
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- Target See all Follistatin (FST) Antibodies
- Follistatin (FST)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Follistatin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
- Brand
- IHC-plus™
- Specificity
- Human Follistatin
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human Follistatin.
Type of Immunogen: Cell extract - Isotype
- IgG
- Top Product
- Discover our top product FST Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (8 - 12 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7434 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100 ng/mL), a concentration of 8-12 μg/mL of this antibody is required. ELISA: To detect Human Follistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (LS-C104465) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Follistatin. Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- Follistatin (FST)
- Alternative Name
- FST / Follistatin (FST Products)
- Background
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Name/Gene ID: FST
Synonyms: FST, Activin-binding protein, Follistatin isoform FST317, Follistatin, Fs - Gene ID
- 10468
- UniProt
- P19883
- Pathways
- Negative Regulation of Hormone Secretion
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