LPAR1 antibody (AA 342-359)

Catalog No. ABIN1101276

Product Details anti-LPAR1 Antibody

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Binding Specificity: AA 342-359

Reactivity: Human, Mouse, Rat, Monkey, Cow, Xenopus laevis, Dog, Hamster, Horse, Pig, Bat, Chicken, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Human lysophosphatidic acid 1 (LPA1) amino acids 342-359 (DRSASSLNHTILAGVHSN)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken, Platypus, Xenopus (100%) Elephant, Rabbit (94%).

Purification: Immunoaffinity purified

Immunogen: Human lysophosphatidic acid 1 (LPA1) amino acids 342-359 (DRSASSLNHTILAGVHSN). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken, Platypus, Xenopus (100%), Elephant, Rabbit (94%).

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: 500 μL sterile water

Concentration: Lot specific

Buffer: TBS,  pH 7.4, containing 50 % glycerol, 0.1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for LPAR1

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Alternative Name: LPAR1 / LPA1 / EDG2 (LPAR1 Products)

Background: Name/Gene ID: LPAR1
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: LPAR1, EDG-2, EDG2, Gpcr26, Rec.1.3, Vzg-1, Ventricular zone gene 1, LPA receptor 1, LPA-1, LPA1, Mrec1.3, VZG1

Gene ID: 1902

UniProt: Q92633

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration