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Trypsin antibody

Reactivity: Cow WB, ELISA, IF, DB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1099706
  • Target See all Trypsin Antibodies
    Trypsin
    Reactivity
    • 12
    • 11
    • 9
    Cow
    Host
    • 27
    • 5
    Rabbit
    Clonality
    • 25
    • 7
    Polyclonal
    Conjugate
    • 20
    • 7
    • 3
    • 2
    This Trypsin antibody is un-conjugated
    Application
    • 25
    • 24
    • 9
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

    Physicochemical characteristics: IgG protein concentration 10 mg/ml. No foreign proteins added.
    Purification
    Antisera
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Trypsin isolated and purified from bovine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Top Product
    Discover our top product Trypsin Primary Antibody
  • Application Notes
    Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/7,000.
    Comment

    Physical form: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.
    Preservative
    Without preservative
    Handling Advice
    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    Storage
    4 °C
  • Target
    Trypsin
    Abstract
    Trypsin Products
    Synonyms
    wu:fb57g08 antibody, zgc:109701 antibody, LOC397853 antibody, Trypsin antibody, trp1 antibody, try1 antibody, trypsin antibody, protease, serine 1 antibody, protease, serine 1 L homeolog antibody, protease, serine, 1 antibody, trypsin antibody, prss1 antibody, prss1.L antibody, Deipr_2253 antibody
    Background
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
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