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Phosphodiesterase II antibody (Biotin)

Reactivity: Cow ELISA, WB, IHC, DB, ICC Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1099699
  • Target See all Phosphodiesterase II products
    Phosphodiesterase II
    Reactivity
    Cow
    Host
    • 4
    Rabbit
    Clonality
    • 4
    Polyclonal
    Conjugate
    • 2
    • 2
    This Phosphodiesterase II antibody is conjugated to Biotin
    Application
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB), Immunocytochemistry (ICC)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

    Physicochemical characteristics: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.2. No foreign proteins added. Marker N-Hydroxysuccinimidobiotin.
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Phosphodiesterase II isolated and purified from calf spleen. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes
    Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/17,000.
    Comment

    Physical form: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

    Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.
    Preservative
    Without preservative
    Handling Advice
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    Storage
    4 °C
  • Target
    Phosphodiesterase II
    Abstract
    Phosphodiesterase II Products
    Background
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
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