AVP
Reactivity: Human
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA (1 microg/ml). IHC (Paraffin-embedded sections) (User test): 4um paraffin sections, dilution 1:50 (Standard protocol), antigen retrieval with glycol-based buffer (available upon request). Immunofluorescence (User test): 1:1000 using Alexa Fluor 488. This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Resuspend in aqua bidest.
Storage
4 °C
Maejima, Kato, Horita, Ueta, Takenoshita, Kobayashi, Shimomura: "The hypothalamus to brainstem circuit suppresses late-onset body weight gain." in: Scientific reports, Vol. 9, Issue 1, pp. 18360, (2019) (PubMed).
Blume, Torner, Liu, Subburaju, Aguilera, Neumann: "Prolactin activates MAP kinase signaling and corticotropin releasing hormone (CRH) transcription in rat hypothalamic neurons." in: Endocrinology, (2008) (PubMed).
Blume, Bosch, Miklos, Torner, Wales, Waldherr, Neumann: "Oxytocin reduces anxiety via ERK1/2 activation: local effect within the rat hypothalamic paraventricular nucleus." in: The European journal of neuroscience, Vol. 27, Issue 8, pp. 1947-56, (2008) (PubMed).