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PLK1 antibody (AA 331-508)

Name: PLK1 antibody (AA 331-508)
Brand: antibodies-online
SKU: ABIN1098105
Price: 511.5 USD
Availability: InStock

PLK1 Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 3C11 unconjugated

Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate. (PLK1 antibody (AA 331-508))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PLK1 antibody (AA 331-508))

ELISA

Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red). (PLK1 antibody (AA 331-508))

FACS

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining. (PLK1 antibody (AA 331-508))

IHC

Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (PLK1 antibody (AA 331-508))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining. (PLK1 antibody (AA 331-508))

IHC

6 images

(3 references)

Catalog No. ABIN1098105

Datasheet as PDF

Target See all PLK1 Antibodies

PLK1 (Polo-Like Kinase 1 (PLK1))
Binding Specificity
22

18

15

12

8

7

5

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 331-508
Reactivity
128

52

48

7

7

5

5

5

5

3

3

3

1

1

1

1
Human
Host
105

22

2

1
Mouse
Compatible Secondaries
Clonality
109

21
Monoclonal
Conjugate
73

6

5

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1
This PLK1 antibody is un-conjugated
Application
98

46

32

28

27

20

17

15

7

4

4

3

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose

PLK1 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Clone

3C11

Isotype

IgG1
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibody
PLK1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 2G12 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibody
PLK1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 4G11 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 Reactivity: Human WB, IHC, IHC (p), IP, FACS Host: Mouse Monoclonal 5C1 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 Reactivity: Human WB, IHC, IHC (p), IP Host: Mouse Monoclonal 1D4 unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 Reactivity: Human, Dog WB, IHC, IHC (p), IP Host: Mouse Monoclonal 6C6 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 304-603) antibody
PLK1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 205-214), (pThr210) antibody
PLK1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 Reactivity: Human, Dog, Monkey WB, IF, IP, FACS Host: Mouse Monoclonal 8C12 unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibody
PLK1 Reactivity: Human WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 Reactivity: Human ELISA, ICC Host: Mouse Monoclonal 1D1 unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Lin, Sun, Wang: "Suppression of Polo like kinase 1 (PLK1) by p21(Waf1) mediates the p53-dependent prevention of caspase-independent mitotic death." in: Cellular signalling, Vol. 23, Issue 11, pp. 1816-23, (2011) (PubMed).

Yoshioka, Kimura, Saio, Era, Okano: "Plk1 is negatively regulated by RNF8." in: Biochemical and biophysical research communications, Vol. 410, Issue 1, pp. 57-61, (2011) (PubMed).

Susilowati, Okamura, Hirota, Shono, Yoshida, Murakami, Tabata, Nagamune, Haneji, Miyake: "Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 57-61, (2011) (PubMed).
Target

PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name

PLK1 (PLK1 Products)

Synonyms

PLK antibody, STPK13 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, stpk13 antibody, Plk antibody, cb525 antibody, cb636 antibody, ik:tdsubc_2d1 antibody, wu:fb37g11 antibody, wu:fb76g03 antibody, xx:tdsubc_2d1 antibody, polo like kinase 1 antibody, polo like kinase 1 S homeolog antibody, polo-like kinase 1 antibody, polo-like kinase 1 (Drosophila) antibody, Serine/threonine-protein kinase plk-1 antibody, PLK1 antibody, plk1.S antibody, plk1 antibody, Plk1 antibody, plk-1 antibody

Background

Description: Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death.
Aliases: PLK,STPK13

Molecular Weight

68kDa

Gene ID

5347

HGNC

5347

UniProt

P53350

Pathways

Cell Division Cycle, M Phase