Zona Radiata Protein antibody
-
- Target
- Zona Radiata Protein
- Reactivity
- Atlantic Salmon
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Specificity
- The monoclonal antibody MN-2B4 binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the b monomer. The antibody also cross-reacts with Zrp from rainbow trout (Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus). The degree of cross-reactivity differs between species and with the methods employed.
- Immunogen
- Immunogen source: Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).
- Clone
- MN-2B4
-
-
- Application Notes
- The monoclonal antibody MN-2B4 works well in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1: 5000. Western blot: 1:100 - 1: 1000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Storage
- 4 °C
-
- Target
- Zona Radiata Protein
- Synonyms
- zrp antibody, zona radiata protein antibody, zrp antibody
- Background
- This package contains one vial of 100 µl antibody solution. The antibody is dissolved in 0.1 M Na-phosphate buffer, 0.15 M NaCl, 1 % BSA, 12.3 mM Na-azide, pH 7.4.
-