Flow cytometry: 1.2 µg/10^6 cells ELISA: 1:200 - 1:400 CELISA: 1:200 - 1:400 For each application a titration should be performed to determine the optimal concentration.
ELISA pair with GM6C3 (detector)
Restrictions
For Research Use only
Buffer
PBS, pH 7.2
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C
Storage Comment
short term: 2 °C - 8 °C, long term: -20 °C
Target
GZMK
(Granzyme K (Granzyme 3, Tryptase II) (GZMK))
TRYP2 antibody, granzyme K antibody, GZMK antibody, Gzmk antibody
Background
Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. GM24C3 was generated by genetic immunisation and reacts with human granzyme K (GrK), a 28 kDa serine protease with trypsin-like specificity.