GZMB antibody

Catalog No. ABIN108719

Product Details anti-GZMB Antibody

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Cell-ELISA (cELISA)

Specificity: GM4C1 recognises granzyme B transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. The antibody does not cross-react with the human granzymes A, K or M (Fi

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding human Granzyme B

Clone: GM-4C1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B (GZMB Products)

Synonyms: CCPI antibody, CGL-1 antibody, CGL1 antibody, CSP-B antibody, CSPB antibody, CTLA1 antibody, CTSGL1 antibody, HLP antibody, SECT antibody, granzyme B antibody, GZMB antibody

Background: Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. GM4C1 was generated by genetic immunisation and reacts with human granzyme B (GrB), a 27 kDa serine protease which cleaves target-cell proteins at specific aspartate residues.

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis