HNRNPU antibody

Catalog No. ABIN108578

Product Details anti-HNRNPU Antibody

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPU antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)

No Cross-Reactivity: Chicken, Lizard

Cross-Reactivity (Details): Mouse, Human, Monkey. Does not react with Chicken, Lizard

Purification: Protein A

Immunogen: RNPs eluted from an oligo (dt) cellulose column

Clone: 3G6

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).ICC/IFICC/IF: 1/100.Flow CytFlow Cyt: Use 1 μg for 106 cells.

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPU antibody (ABIN108578)

Ma, Matsunaga, Morimoto, Sakashita, Urano, Uchiyama, Fukui: "The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles." in: Journal of cell science, Vol. 124, Issue Pt 3, pp. 394-404, (2011) (PubMed).

Pare, López-Orozco, Hobman: "MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies." in: Biochemical and biophysical research communications, Vol. 414, Issue 1, pp. 259-64, (2011) (PubMed).

Raju, Fukuda, López-Iglesias, Göritz, Visa, Percipalle: "In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements." in: Molecular biology of the cell, Vol. 22, Issue 11, pp. 1864-77, (2011) (PubMed).

Tserel, Runnel, Kisand, Pihlap, Bakhoff, Kolde, Peterson, Vilo, Peterson, Rebane: "MicroRNA expression profiles of human blood monocyte-derived dendritic cells and macrophages reveal miR-511 as putative positive regulator of Toll-like receptor 4." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26487-95, (2011) (PubMed).

Hadian, Vincendeau, Mäusbacher, Nagel, Hauck, Ueffing, Loyter, Werner, Wolff, Brack-Werner: "Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33384-91, (2009) (PubMed).

Uchimura, Ichimura, Uwada, Tachibana, Sugahara, Nakao, Saitoh: "Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23180-90, (2006) (PubMed).

Ahmad, Lingrel: "Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonucleoproteins and acetyltransferases." in: Biochemistry, Vol. 44, Issue 16, pp. 6276-85, (2005) (PubMed).

Dreyfuss, Choi, Adam: "Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies." in: Molecular and cellular biology, Vol. 4, Issue 6, pp. 1104-14, (1984) (PubMed).

Target Details for HNRNPU

Target: HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A) (HNRNPU))

Alternative Name: hnRNP U/p120 (HNRNPU Products)

Synonyms: AA408410 antibody, AI256620 antibody, AL024194 antibody, AL024437 antibody, AW557595 antibody, C86794 antibody, Hnrpu antibody, SAFA antibody, Sp120 antibody, hnRNP U antibody, SN1 antibody, HNRPU antibody, SAF-A antibody, U21.1 antibody, saf-a antibody, heterogeneous nuclear ribonucleoprotein U antibody, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) S homeolog antibody, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) antibody, Hnrnpu antibody, HNRNPU antibody, hnrnpu.S antibody

Background: Synonyms:
Heterogeneous nuclear ribonucleoprotein U antibody,hnRNP U protein antibody,HNRNPU antibody,hnRNPUprotein antibody,HNRPU antibody,p120 antibody,p120 nuclear protein antibody,pp120 antibody,SAF Aantibody,SAF-A antibody,SAFA antibody,Scaffold attachment factor A antibody,U21.1antibody

Gene ID: 3192, 51810

OMIM: 602869

UniProt: P31943