HNRNPL antibody

Catalog No. ABIN108560

Product Details anti-HNRNPL Antibody

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Reactivity: Human, Mouse, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Human hnRNP F/H

Purification: Protein A

Clone: 1G11

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try:IHC-P: Use a concentration of 1 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB: Use a concentration of 1 μg/mL. Detects a band of approximately 53 kDa (hnRNP F), 56 kDa (hnRNP H). (predicted molecular weight: hnRNP F = 49.8 kDa, hnRNP H = 53.9 kDa).

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPL antibody (ABIN108560)

Marzano, Santini, Rossi, Zucchelli, DAlessandro, Marchetti, Mingardi, Stagni, Barilà, Urbani: "Proteomic profiling of ATM kinase proficient and deficient cell lines upon blockage of proteasome activity." in: Journal of proteomics, Vol. 75, Issue 15, pp. 4632-46, (2012) (PubMed).

Tsai, Wang, Huang, Waterman: "Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 4, pp. M110.007385, (2011) (PubMed).

Millevoi, Decorsière, Loulergue, Iacovoni, Bernat, Antoniou, Vagner: "A physical and functional link between splicing factors promotes pre-mRNA 3' end processing." in: Nucleic acids research, Vol. 37, Issue 14, pp. 4672-83, (2009) (PubMed).

Paronetto, Achsel, Massiello, Chalfant, Sette: "The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x." in: The Journal of cell biology, Vol. 176, Issue 7, pp. 929-39, (2007) (PubMed).

Target Details for HNRNPL

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Alternative Name: hnRNP F+H  (HNRNPL Products)

Synonyms: HNRPL antibody, hnRNP-L antibody, zgc:55429 antibody, HNRNPL antibody, hnrpl antibody, C79783 antibody, D830027H13Rik antibody, Hnrpl antibody, hnrnp-L antibody, heterogeneous nuclear ribonucleoprotein L antibody, HNRNPL antibody, hnrpl antibody, hnrnpl antibody, Hnrnpl antibody

Background: Synonyms:
Heterogeneous nuclear ribonucleoprotein F antibody,Heterogeneous nuclear ribonucleoprotein H1antibody,hnRNP F antibody,hnRNP H antibody,hnRNPF antibody,hnRNPH antibody,HNRNPH1 antibody,HNRPFantibody,HNRPH antibody,HNRPH1 antibody,mcs94 1 antibody,Nucleolin like protein antibody,Nucleolin likeprotein mcs94 1 antibody

Gene ID: 3185, 3187

UniProt: P52597, P31943