DDX20 antibody (AA 368-548)

Catalog No. ABIN108547

Product Details anti-DDX20 Antibody

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Binding Specificity: AA 368-548

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: Protein A

Immunogen: Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548)

Clone: 12H12

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try:WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).Flow Cyt: Use 1 μg for 106 cells.ICC/IF: 1/50.

Comment:

Myeloma, fusion partners: SP2/0,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-DDX20 antibody (ABIN108547)

Mahmoudi, Henriksson, Weibrecht, Smith, Söderberg, Strömblad, Wiman, Farnebo: "WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies." in: PLoS biology, Vol. 8, Issue 11, pp. e1000521, (2010) (PubMed).

Bradrick, Gromeier: "Identification of gemin5 as a novel 7-methylguanosine cap-binding protein." in: PLoS ONE, Vol. 4, Issue 9, pp. e7030, (2009) (PubMed).

Castelló, Izquierdo, Welnowska, Carrasco: "RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage." in: Journal of cell science, Vol. 122, Issue Pt 20, pp. 3799-809, (2009) (PubMed).

Walker, Rajendra, Saieva, Fuentes, Pellizzoni, Matera: "SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain." in: Human molecular genetics, Vol. 17, Issue 21, pp. 3399-410, (2008) (PubMed).

Morency, Sabra, Catez, Texier, Lomonte: "A novel cell response triggered by interphase centromere structural instability." in: The Journal of cell biology, Vol. 177, Issue 5, pp. 757-68, (2007) (PubMed).

Hamamoto, Nishitsuji, Amagasa, Kannagi, Masuda: "Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo." in: Journal of virology, Vol. 80, Issue 12, pp. 5670-7, (2006) (PubMed).

Zhang, Xing, Rossoll, Wichterle, Singer, Bassell: "Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 33, pp. 8622-32, (2006) (PubMed).

Charroux, Pellizzoni, Perkinson, Shevchenko, Mann, Dreyfuss: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, (2000) (PubMed).

Target Details for DDX20

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Alternative Name: Gemin 3 (DDX20 Products)

Synonyms: DDX20 antibody, DP103 antibody, GEMIN3 antibody, dp103 antibody, Dp103 antibody, si:dkey-193l3.3 antibody, wu:fb16g08 antibody, wu:fb59a11 antibody, DEAD-box helicase 20 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody, DDX20 antibody, ddx20 antibody, Ddx20 antibody

Background: Synonyms:
Component of gems 3 antibody,DDX 20 antibody,DDX20 antibody,DDX20_HUMAN antibody,DEAD (Asp Glu AlaAsp) box polypeptide 20 antibody,DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody,DEAD box protein 20antibody,DEAD box protein DP 103 antibody,DEAD box protein DP103 antibody,DEAD-box protein DP103antibody,DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody,DEAD/H (Asp Glu Ala Asp/His) boxpolypeptide 20 antibody,DEAD/H BOX 20 antibody,DKFZP434H052 antibody,DP 103 antibody,DP103 antibody,Gemin-3antibody,Gemin3 antibody,Probable ATP dependent RNA helicase DDX20 antibody,Probable ATP-dependent RNA helicaseDDX20 antibody,SMN interacting protein antibody

Gene ID: 66603

OMIM: 606168

Pathways: Ribonucleoprotein Complex Subunit Organization