PABP antibody

Catalog No. ABIN108538

Product Details anti-PABP Antibody

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reactivity: Human, Rabbit, Chicken, Xenopus laevis, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PABP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: Protein G

Immunogen: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI

Clone: 10E10

Isotype: IgG2b

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1 μg for 106 cells

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-PABP antibody (ABIN108538)

Gorgoni, Richardson, Burgess, Anderson, Wilkie, Gautier, Martins, Brook, Sheets, Gray: "Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7844-9, (2011) (PubMed).

Unsworth, Raguz, Edwards, Higgins, Yagüe: "mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3370-80, (2010) (PubMed).

Görlach, Burd, Dreyfuss: "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity." in: Experimental cell research, Vol. 211, Issue 2, pp. 400-7, (1994) (PubMed).

Target Details for PABP

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Alternative Name: PABP (PABPC1 Products)

Synonyms: PAB1 antibody, PABP antibody, PABP1 antibody, PABPC2 antibody, PABPL1 antibody, Pabp1 antibody, PabpI antibody, Pabpl1 antibody, ePAB antibody, Pabp antibody, pab1 antibody, pabp antibody, pabp1 antibody, pabpc1 antibody, pabpc2 antibody, PABPC1 antibody, wu:fb16a02 antibody, wu:fi19b08 antibody, wu:fj12d09 antibody, wu:fj61f06 antibody, zgc:109879 antibody, zgc:77608 antibody, poly(A) binding protein cytoplasmic 1 antibody, poly(A) binding protein, cytoplasmic 1 antibody, poly(A) binding protein, cytoplasmic 1 S homeolog antibody, poly(A) binding protein cytoplasmic 1 like antibody, poly(A) binding protein, cytoplasmic 1 L homeolog antibody, poly(A) binding protein, cytoplasmic 1a antibody, poly A binding protein, cytoplasmic 1 b antibody, PABPC1 antibody, Pabpc1 antibody, pabpc1.S antibody, pabpc1 antibody, PABPC1L antibody, pabpc1.L antibody, pabpc1a antibody, pabpc1b antibody

Background: Synonyms:
PAB 1 antibody,PAB1 antibody,PABP 1 antibody,PABP1 antibody,PABPC 1 antibody,PABPC1antibody,PABPC2 antibody,PABPL1 antibody,Poly A binding protein 1 antibody,Poly A binding protein cytoplasmic1 antibody,Polyadenylate binding protein 1 antibody

Gene ID: 117282

OMIM: 604679

UniProt: P22626

Pathways: SARS-CoV-2 Protein Interactome