VSV-g Tag antibody (AA 501-511)

Catalog No. ABIN108207

Supplier Product No.: 600-401-386

Product Details anti-VSV-g Tag Antibody

Target: VSV-g Tag (VSV-G Epitope Tag)

Binding Specificity: AA 501-511

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VSV-g Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-386

Supplier: Rockland

Purpose: VSV-G Antibody

Sequence: YTDIEMNRLG K

Cross-Reactivity (Details): This affinity purified antibody is directed against VSV-G protein.

Characteristics: Synonyms: Rabbit Anti-VSV-G Epitope Tag Antibody, Rabbit Anti VSV-G Epitope Tag Antibody, Rabbit Anti-VSV-G Tag Antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:500

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. This product is suitable for immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200.   At higher concentrations, a filamentous background staining may be present.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:50,000 - 1:100,000

IF Microscopy Dilution: 1:200 - 1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-VSV-g Tag antibody (ABIN108207)

Muñoz-Álvarez, Altomonte, Laitinen, Ziegler, Steiger, Esposito, Schmid, Ebert: "PET imaging of oncolytic VSV expressing the mutant HSV-1 thymidine kinase transgene in a preclinical HCC rat model." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 4, pp. 728-36, (2016) (PubMed).

Target Details for VSV-g Tag

Target: VSV-g Tag (VSV-G Epitope Tag)

Alternative Name: VSV-G (VSV-g Tag Products)

Target Type: Tag

Background: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  VSV-G or vesicular stomatitis virus glycoprotein is found within the pseudo lentiviral cloning vector pH CMV-VSV-G.