Urm1 antibody

Catalog No. ABIN108085

Supplier Product No.: 200-401-443

Product Details anti-Urm1 Antibody

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urm1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-443

Supplier: Rockland

Purpose: Urm1 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-Urm1 Antibody, C9orf74 antibody, Chromosome 9 open reading frame 74 antibody, MGC2668 antibody, RP11 339B21.4 antibody, Ubiquitin Related Modifier 1 antibody, Ubiquitin related modifier 1 homolog antibody, Urm 1 antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Urm1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Urm1

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Alternative Name: URM1 (Urm1 Products)

Background: Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Urm1 is a newly identified ubiquitin related modifier.  Urm 1 is a 99-amino acid protein terminated with glycine-glycine.  Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.

Gene ID: 854809

UniProt: P40554