Uricase (ABC3735) antibody (Biotin)

Catalog No. ABIN108081

Supplier Product No.: 200-106-092-0100

Product Details

Target: Uricase (ABC3735)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Supplier Product No.: 200-106-092-0100

Supplier: Rockland

Purpose: Anti-Uricase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

Characteristics: Synonyms: goat anti-uricase antibody, biotin conjugated goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

Purification: Anti-Uricase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Uricase [Bacillus species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Uricase Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 μg of Uricase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:53,000 of the reconstitution concentration is suggested for Anti-Uricase Antibody.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Uricase (ABC3735)

Alternative Name: ABC3735

Target Type: Dinucleotide

Background: Background: Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH 's. Anti-Uricase Antibody is ideal for investigators in Cell Biology and Microbiology research.

Gene ID: 3202700

NCBI Accession: YP_177228

UniProt: Q5WBJ3