Uricase (ABC3735) antibody

Catalog No. ABIN108080

Supplier Product No.: 200-101-092-0100

Product Details

Target: Uricase (ABC3735)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Supplier Product No.: 200-101-092-0100

Supplier: Rockland

Purpose: Uricase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

Characteristics: Synonyms: goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

Purification: Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Uricase [Bacillus species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Uricase Antibody has been tested by western blot and is assayed against 1.0 μg of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Uricase (ABC3735)

Alternative Name: ABC3735

Target Type: Dinucleotide

Background: Background: Uricase, or urate oxidase, catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Urate oxidase is found in nearly all organisms, from bacteria to mammals, and plays different metabolic roles, depending on its host organism. Humans are the only animal that are unable to break down uric acid to allantoin. This is because humans do not have the necessary enzyme uricase. Humans do have a gene for urate oxidase, but it is nonfunctional. Thus uric acid is the end product of animal products in humans. This leads to an increased possibility of an accumulation of uric acid in the body when animal products are eaten. Excessive concentration of uric acid in the blood stream leads to gout. It has been proposed that the loss of urate oxidase gene expression has been advantageous to primates, since uric acid is a powerful antioxidant and scavenger of singlet oxygen and radicals. Its presence provides the body with protection from oxidative damage, thus prolonging life and decreasing age-specific cancer rates.

Gene ID: 3202700

NCBI Accession: YP_177228

UniProt: Q5WBJ3