Urease antibody

Catalog No. ABIN108075

Supplier Product No.: 200-4182-0100

Product Details anti-Urease Antibody

Target: Urease (URE)

Reactivity: Jack Bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-4182-0100

Supplier: Rockland

Purpose: Urease Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Characteristics: Synonyms: rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Purification: Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Urease [Jack Bean]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 μg of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease (URE Products)

Background: Background: Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate (ammonia and carbonic acid). Ureases are found in numerous bacteria, fungi, algae, invertebrates, plants, in soils, as a soil enzyme. Due to the catalytic activity producing ammonia the pH of the environment increases. Ureases, when bacterial, can be a mode for the pathogens of many medical conditions such as hepatic encephalopathy, hepatic coma, urinary stones, and peptic ulcers. Urea is found naturally in the environment as well as artificially introduced. Anti-Urease antibody is ideal for researchers interested in ecosystems, microbes, and gut bacteria.

UniProt: P07374