Urease antibody

Catalog No. ABIN108075

Product Details anti-Urease Antibody

Target: Urease (URE)

Reactivity: Jack Bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.

Purity: Anti-UREASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Endotoxin Level: Low Endotoxin : No

Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-UREASE Antibody has been assayed against 1.0 µg of Urease [Jack Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:60.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease (URE Products)

Synonyms: F12B7.10 antibody, F12B7_10 antibody, urease antibody, urease antibody, URE antibody, CNH01900 antibody

Background: Synonyms: Urea Amidohydrolase antibody

UniProt: P07374