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Urease antibody

Rockland 200-4182-0100 URE Reactivity: Jack Bean WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN108075
Supplier Product No.: 200-4182-0100
  • Target See all Urease (URE) products
    Urease (URE)
    Reactivity
    • 9
    • 2
    Jack Bean
    Host
    • 11
    Rabbit
    Clonality
    • 11
    Polyclonal
    Conjugate
    • 5
    • 5
    • 1
    This Urease antibody is un-conjugated
    Application
    • 11
    • 9
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    200-4182-0100
    Supplier
    Rockland
    Purpose
    Urease Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
    Characteristics
    Synonyms: rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
    Purification
    Anti-Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Urease [Jack Bean]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Urease Antibody has been tested in western blot and suitable against 1.0 μg of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Urease (URE)
    Alternative Name
    Urease (URE Products)
    Background
    Background: Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Urease catalyzes the hydrolysis of urea to produce ammonia and carbamate (ammonia and carbonic acid). Ureases are found in numerous bacteria, fungi, algae, invertebrates, plants, in soils, as a soil enzyme. Due to the catalytic activity producing ammonia the pH of the environment increases. Ureases, when bacterial, can be a mode for the pathogens of many medical conditions such as hepatic encephalopathy, hepatic coma, urinary stones, and peptic ulcers. Urea is found naturally in the environment as well as artificially introduced. Anti-Urease antibody is ideal for researchers interested in ecosystems, microbes, and gut bacteria.
    UniProt
    P07374
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