Trypsinogen antibody

Catalog No. ABIN107877

Supplier Product No.: 200-4180-0100

Product Details anti-Trypsinogen Antibody

Target: Trypsinogen

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsinogen antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Supplier Product No.: 200-4180-0100

Supplier: Rockland

Purpose: Trypsinogen Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].

Characteristics: Synonyms: rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody

Purification: Anti-Trypsinogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsinogen [Bovine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Trypsinogen Antibody has been tested by ELIDA and dot blot and is assayed against 1.0 μg of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Trypsinogen

Target: Trypsinogen

Abstract: Trypsinogen Products

Background: Background: Trypsinogen, is a precursor to trypsin and a digestive enzyme. It functions as storage of an inactive form of trypsin and kept in the pancreas then released in significant amount, along with other enzymes, when required for protein digestion. Serum trypsinogen is measured using a blood test. Diseases associated with Trypsinogen high levels or mutations are acute pancreatitis and cystic fibrosis. Anti-Trypsinogen Antibody can be useful for researchers interested in studying the serum or pancreas.

Gene ID: 282603

UniProt: Q29463