Trypsinogen antibody

Catalog No. ABIN107877

Product Details anti-Trypsinogen Antibody

Target: Trypsinogen

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsinogen antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.

Purity: Anti-TRYPSINOGEN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].

Endotoxin Level: Low Endotoxin : No

Immunogen: Trypsinogen [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: TRYPSINOGEN Antibody has been assayed against 1.0 µg of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Trypsinogen

Target: Trypsinogen

Abstract: Trypsinogen Products

Synonyms: PRSS2 antibody, LOC397853 antibody, Trypsin antibody, LOC100302368 antibody, LOC446096 antibody, tryp antibody, tryl antibody, prss2a antibody, wu:fb61f11 antibody, protease, serine 3 antibody, protease, serine 1 L homeolog antibody, trypsinogen antibody, protease, serine, 59, tandem duplicate 1 antibody, PRSS3 antibody, prss1.L antibody, LOC100302368 antibody, LOC446096 antibody, tryp antibody, prss59.1 antibody, LOC100379576 antibody

Background: Synonyms: Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody

Gene ID: 282603

UniProt: Q29463