Trypsin Inhibitor antibody
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- Target See all Trypsin Inhibitor products
- Trypsin Inhibitor
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Reactivity
- Soybean
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Trypsin Inhibitor antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 200-401-079
- Supplier
- Rockland
- Purpose
- Trypsin Inhibitor Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean].
- Characteristics
- Synonyms: rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
- Purification
- Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Trypsin Inhibitor [Soybean]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Application Note: Soybean trypsin inhibitor antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Trypsin Inhibitor [Soybean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Trypsin Inhibitor
- Abstract
- Trypsin Inhibitor Products
- Background
- Background: Trypsin inhibitor antibody is specific for soybean trypsin inhibitor. Soybean trypsin inhibitor inhibits trypsin, but also chymotrypsin and plasmin at a reduced level. SBTI has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases.
- Gene ID
- 547831
- UniProt
- P01070
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