TGFB1 antibody (AA 279-390)
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- Target See all TGFB1 Antibodies
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
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Binding Specificity
- AA 279-390
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Reactivity
- Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TGFB1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-AL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQV LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS
- Specificity
- It has been selected for its ability to recognize TGFb1 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
- TGFb1 (AA 279-390)
- Isotype
- IgG
- Top Product
- Discover our top product TGFB1 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant TGFb1 (Ala279~Ser390) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- Alternative Name
- Transforming Growth Factor Beta 1 (TGFb1) (TGFB1 Products)
- Synonyms
- CED antibody, DPD1 antibody, LAP antibody, TGFB antibody, TGFbeta antibody, TGF-beta antibody, TGF-BETA-1 antibody, TGF-beta5 antibody, ced antibody, dpd1 antibody, lap antibody, tgf-beta antibody, tgfb antibody, tgfb5 antibody, tgfbeta antibody, TGF-beta1 antibody, TGFbeta1 antibody, Tgfb antibody, Tgfb-1 antibody, ai39657 antibody, tgfb1 antibody, wu:fb13a07 antibody, xx:ai39657 antibody, TGFB1 antibody, csd antibody, cdb1 antibody, cdg2 antibody, csd1 antibody, csd2 antibody, csd3 antibody, ebmd antibody, lcd1 antibody, bigh3 antibody, cdgg1 antibody, betaig-h3 antibody, TGFB4 antibody, transforming growth factor beta 1 antibody, transforming growth factor beta-1 antibody, transforming growth factor beta 1 L homeolog antibody, transforming growth factor, beta 1 antibody, transforming growth factor, beta 1a antibody, transforming growth factor beta induced L homeolog antibody, TGFB1 antibody, Tgfb1 antibody, tgfb1.L antibody, tgfb1a antibody, tgfbi.L antibody
- Pathways
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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