NFkB antibody (AA 40-365)
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- Target See all NFkB Antibodies
- NFkB (Nuclear Factor kappa B (NFkB))
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Binding Specificity
- AA 40-365
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NFkB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NFkB. It has been selected for its ability to recognize NFkB in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Pig, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Nuclear Factor Kappa B (NFkB) corresdonding to Pro40~Met365 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product NFkB Primary Antibody
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- Application Notes
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Western blotting: 0.5-5 μg/mL
Immunohistochemistry: 5-50 μg/mL
Immunocytochemistry: 5-50 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- NFkB (Nuclear Factor kappa B (NFkB))
- Alternative Name
- Nuclear Factor Kappa B (NFkB Products)
- Synonyms
- EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, NF-kappaB1 antibody, p50/p105 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, NFKB1 antibody, Nfkb1 antibody
- Background
- NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit
- Pathways
- Ubiquitin Proteasome Pathway, S100 Proteins
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