Myosin IB antibody (AA 779-1040)

Catalog No. ABIN1078387

Product Details anti-Myosin IB Antibody

Target: Myosin IB (MYO1B)

Binding Specificity: AA 779-1040

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin IB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: MGHHHHHHSG S- CK EAATTIAAYW HGTQARRELK RLKEEARRKH AVAVIWAYWL GLKVRREYRK FFRANAGKKI YEFTLQRIVQ KYLLEMKNKM PSLSPIDKNW PSRPYLFLDS THKELKRIFH LWRCKKYRDQ FTDQQKLIYE EKLEASELFK DKKALYPSSV GQPFQGAYLE INKNPKYKKL KDAIEEKIII AEVVNKINRA NGKSTSRIFL LTNNNLLLAD QKSGQIKSEV PLVDVTKVSM SSQNDGFFAV HLKEGSEAAS KGDFLFSSDH

Specificity: It has been selected for its ability to recognize MYO1B in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant MYO1B expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against MYO1B.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant MYO1B (Cys779~His1040) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Myosin IB

Target: Myosin IB (MYO1B)

Abstract: MYO1B Products

Synonyms: MYO1B antibody, DKEY-242N5.2 antibody, myo1b2 antibody, myo1bl1 antibody, myo1bl2 antibody, si:busm1-60j23.1 antibody, wu:fb39h10 antibody, wu:fc46c09 antibody, zgc:110446 antibody, zgc:113187 antibody, myr1 antibody, AA406997 antibody, myosin-1b antibody, Myr1 antibody, myosin IB antibody, myosin Ib antibody, MYO1B antibody, myo1b antibody, DICPUDRAFT_46917 antibody, Myo1b antibody