Ecotin (AA 21-162) antibody
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- Target
- Ecotin
- Binding Specificity
- AA 21-162
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Reactivity
- Various Species
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Ecotin (ECO)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against ECO. It has been selected for its ability to recognize ECO in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Ecotin (ECO) corresdonding to Ala21~Arg162 with N-terminal His Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Ecotin
- Synonyms
- 2 antibody, EAR-13 antibody, EAR-2 antibody, ECP antibody, ECP 2 antibody, Rnase2 antibody, EAR antibody, R1 antibody, R7 antibody, Rnase3 antibody, RNS3 antibody, eosinophil-associated, ribonuclease A family, member 2 antibody, eosinophil-associated, ribonuclease A family, member 11 antibody, ribonuclease A family member 3 antibody, Ear2 antibody, Ear11 antibody, RNASE3 antibody
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