Calmodulin,CAM antibody (AA 1-149)

Catalog No. ABIN1077890

Product Details anti-Calmodulin,CAM Antibody

Target: Calmodulin,CAM

Binding Specificity: AA 1-149

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calmodulin,CAM antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: MGHHHHHHSG S- MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

Specificity: It has been selected for its ability to recognize CAM in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant CAM expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CAM.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant CAM (Met1~Lys149) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Calmodulin,CAM

Target: Calmodulin,CAM

Alternative Name: Calmodulin (CAM) (Calmodulin,CAM Products)

Synonyms: CALM2 antibody, CaMA antibody, R75142 antibody, PHKD antibody, PHKD3 antibody, calmodulin 1 antibody, calmodulin 2 antibody, calmodulin 3 antibody, CALM1 antibody, Calm2 antibody, CALM2 antibody, Calm3 antibody, CALM3 antibody