Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Calmodulin,CAM antibody (AA 1-149)

Reactivity: Chicken WB, ICC, ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1077888
  • Target See all Calmodulin,CAM Antibodies
    Calmodulin,CAM
    Binding Specificity
    AA 1-149
    Reactivity
    • 2
    • 1
    Chicken
    Host
    • 3
    Rabbit
    Clonality
    • 3
    Polyclonal
    Conjugate
    • 1
    • 1
    • 1
    This Calmodulin,CAM antibody is un-conjugated
    Application
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequence
    MGHHHHHHSG S-MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
    Specificity
    It has been selected for its ability to recognize CAM in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Recombinant CAM expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CAM.
    Isotype
    IgG
    Top Product
    Discover our top product Calmodulin,CAM Primary Antibody
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Comment

    Content: The quality control contains recombinant CAM (Met1~Lys149) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    Calmodulin,CAM
    Alternative Name
    Calmodulin (CAM) (Calmodulin,CAM Products)
    Synonyms
    CALM2 antibody, CaMA antibody, R75142 antibody, PHKD antibody, PHKD3 antibody, calmodulin 1 antibody, calmodulin 2 antibody, calmodulin 3 antibody, CALM1 antibody, Calm2 antibody, CALM2 antibody, Calm3 antibody, CALM3 antibody
You are here:
Support