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Apotransferrin antibody

Reactivity: Human WB, ELISA, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1077830
  • Target See all Apotransferrin Antibodies
    Apotransferrin
    Reactivity
    • 5
    • 2
    • 2
    • 2
    Human
    Host
    • 11
    Rabbit
    Clonality
    • 11
    Polyclonal
    Conjugate
    • 6
    • 4
    • 1
    This Apotransferrin antibody is un-conjugated
    Application
    • 8
    • 8
    • 5
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    It has been selected for its ability to recognize apoTf in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Native Protein apoTfTransferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Human transferrin is encoded by the TF gene. Transferrin glycoproteins bind iron very tightly, but reversibly. When a transferrin protein loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and, as a consequence, is transported into the cell in a vesicle by receptor-mediated endocytosis. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. A decreased plasma transferrin can occur in iron overload diseases and protein malnutrition. An absence of transferrin results from a rare genetic disorder known as atransferrinemia, a condition characterized by anemia and hemosiderosis in the heart and liver that leads to many complications, including heart failure.
    The antibody is a rabbit polyclonal antibody raised against apoTf.
    Isotype
    IgG
    Top Product
    Discover our top product Apotransferrin Primary Antibody
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    Apotransferrin
    Alternative Name
    Apotransferrin (ApoTf) (Apotransferrin Products)
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