Actin antibody

Catalog No. ABIN1077685

Product Details anti-Actin Antibody

Target: Actin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Sequence: MGHHHHHHSG SEF-MDDDIAA LVVDNGSGMC KAGFAGDDAP RAVFPSIVGR PRHQGVMVGM GQKDSYVGDE AQSKRGILTL KYPIEHGIVT NWDDMEKIWH HTFYNELRVA PEEHPVLLTE APLNPKANRE KMTQIMFETF NTPAMYVAIQ AVLSLYASGR TTGIVMDSGD GVTHTVPIYE GYALPHAILR LDLAGRDLTD YLMKILTERG YSFTTTAERE IVRDIKEKLC YVALDFEQEM ATAASSSSLE KSYELPDGQV ITIGNERFRC PEALFQPSFL GMESCGIHET TFNSIMKCDV DIRKDLYANT VLSGGTTMYP GIADRMQKEI TALAPSTMKI KIIAPPERKY SVWIGGSILA SLSTFQQMWI SKQEYDESGP SIVHRKCF

Specificity: It has been selected for its ability to recognize Actin in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: The antibody is a rabbit polyclonal antibody raised against Actin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN1077685)

Yende, Tyagi: "Influence of signaling kinases on functional dynamics of nuclear receptor CAR." in: Molecular and cellular biochemistry, Vol. 461, Issue 1-2, pp. 127-139, (2020) (PubMed).

Target Details for Actin

Target: Actin

Abstract: Actin Products

Synonyms: Act57 antibody, Act57A antibody, Act57b antibody, Actin antibody, BEST:RE38067 antibody, C antibody, CG10067 antibody, CG30294 antibody, Dmel\\CG10067 antibody, M antibody, T10 antibody, act 57A antibody, act57A antibody, act57B antibody, act57b antibody, actin antibody, anon-EST:CL2c12 antibody, 143060_f_at antibody, CG7478 antibody, D antibody, Dmel\\CG7478 antibody, act 79B antibody, act79B antibody, mus79B antibody, Act(88F) antibody, Act88-F antibody, Act88f antibody, CG5178 antibody, Dmel\\CG5178 antibody, E antibody, Ifm(3)7 antibody, act 88F antibody, act88F antibody, rsd antibody, Act87e antibody, CG18290 antibody, Dmel\\CG18290 antibody, act 87E antibody, act87E antibody, mus87E antibody, Act88F antibody, Dsim\\GD19025 antibody, GD19025 antibody, dsim_GLEANR_2829 antibody, 42A antibody, A antibody, AFFX-Dros-ACTIN_M_r_at antibody, Act antibody, Act42 antibody, Act42a antibody, Actin/BAP47 antibody, BAP47 antibody, CG12051 antibody, Dmel\\CG12051 antibody, act 42A antibody, act42A antibody, cytoplasmic antibody, Actin 57B antibody, Actin 79B antibody, Actin 88F antibody, Actin 87E antibody, Actin-like protein C08B11.6 antibody, hypothetical protein antibody, ACT_COLSC Actin (ISS) antibody, Actin 42A antibody, actin gamma 1 antibody, actin antibody, Actin, gamma 1 antibody, si:ch211-241j12.3 antibody, Act57B antibody, Act79B antibody, Act88F antibody, Act87E antibody, arp-6 antibody, Dsim\Act88F antibody, ACTIN antibody, Act42A antibody, ACTG1 antibody, LOC548170 antibody, Bm1_16810 antibody, IDA5 antibody, LOC100342017 antibody, si:ch211-241j12.3 antibody

Molecular Weight: 43.26 kDa