POMC antibody (AA 27-235)

Catalog No. ABIN1077682

Product Details anti-POMC Antibody

Target: POMC (Proopiomelanocortin (POMC))

Binding Specificity: AA 27-235

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against POMC. It has been selected for its ability to recognize POMC in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: POMC(Trp27-Gln235)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References for anti-POMC antibody (ABIN1077682)

Muhsin, Bentley, Bai, Goldsworthy, Cox: "A novel mutation in the mouse Pcsk1 gene showing obesity and diabetes." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 31, Issue 1-2, pp. 17-29, (2020) (PubMed).

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: Proopiomelanocortin POMC (POMC Products)

Synonyms: MSH antibody, ACTH antibody, pomc antibody, SI:bZ36D5.1 antibody, SI:bZ36D5.4 antibody, si:bz36d5.2 antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, NPP antibody, POC antibody, Pomc2 antibody, proopiomelanocortin a antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, pomca antibody, Pomc antibody, POMC antibody

Background: Alternative Names: POC, NPP, ProOMC, ACTH, Pro-OMC, Pro Opiomelanocortin, Corticotropin-lipotropin, Corticotropin, Adrenocorticotropic hormone

Molecular Weight: 25 kDa

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour