TLK1 antibody (AA 730-755)

Catalog No. ABIN107632

Supplier Product No.: 600-401-469

Product Details anti-TLK1 Antibody

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Binding Specificity: AA 730-755

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-469

Supplier: Rockland

Purpose: Tlk1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Tlk-1 protein.

Characteristics: Synonyms: rabbit anti-TLK1 antibody, TLK-1, TLK 1, PKU-beta antibody, serine threonine protein kinase antibody, Serine/threonine protein kinase tousled like 1 antibody, Tousled like kinase 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract.  This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.60 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: TLK1 (TLK1 Products)

Background: Background: Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent.  Tlk-1 heterodimerizes with Tlk-2.  Multiple isoforms of the protein have been detected.  Mature Tlk-1 is reported to have an apparent molecular weight of ~86 kDa, ~89 kDa and ~64 kDa corresponding to isoforms 1, 2 and 3 respectively.

Gene ID: 9874, 33636698

UniProt: Q9UKI8