Concentration Definition: by UV absorbance at 280 nm
Immunogen
This antibody was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli. Immunogentype:Recombinant
TYMS
Reactivity: Human, Mouse, Rat
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
THYMIDYLATE SYNTHASE antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.