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SUMO1 antibody

Rockland 200-401-441 SUMO1 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN107152
Supplier Product No.: 200-401-441
  • Target See all SUMO1 Antibodies
    SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
    Reactivity
    • 93
    • 78
    • 73
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 98
    • 32
    • 3
    • 1
    • 1
    Rabbit
    Clonality
    • 92
    • 44
    Polyclonal
    Conjugate
    • 69
    • 10
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SUMO1 antibody is un-conjugated
    Application
    • 110
    • 48
    • 46
    • 37
    • 32
    • 27
    • 26
    • 23
    • 16
    • 9
    • 8
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    200-401-441
    Supplier
    Rockland
    Purpose
    SUMO Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
    Characteristics
    Synonyms: rabbit anti-SUMO antibody, GAP modifying protein 1 antibody, GMP 1 antibody, GMP1 antibody, PIC 1 antibody, PIC1 antibody, SENP2 antibody, Sentrin 1 antibody, Sentrin antibody, Small ubiquitin related modifier 1 antibody
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
    Top Product
    Discover our top product SUMO1 Primary Antibody
  • Application Notes

    Application Note: This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 1:2,000 dilution is recommended.  An 11.6 kDa band corresponding to human SUMO is detected.  Most human cell lysates can be used as a positive control without induction or stimulation.  For ELISA a 1:4,000 to 1:20,000 dilution is recommended.   Researchers should determine optimal titers for other applications.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    5.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Meyer, Shah, Zhang, Rohrs, Rao: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).

  • Target
    SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
    Alternative Name
    SUMO1 (SUMO1 Products)
    Background
    Background: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-a, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50 % sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18 % homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with b-sheets wrapped around an a-helix.
    Gene ID
    7341
    NCBI Accession
    NP_001005781
    UniProt
    P63165
    Pathways
    M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
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