Reactivity: E. coli
WB, IP, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
This product has been assayed against 1.0 µg of Sucrose Phosphorylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:115,000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5,000 - 1:20,000 Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5,000
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.