Sucrose Phosphorylase antibody
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- Target See all Sucrose Phosphorylase products
- Sucrose Phosphorylase
- Reactivity
- E. coli
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Sucrose Phosphorylase antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Supplier Product No.
- 200-101-231-0100
- Supplier
- Rockland
- Purpose
- Sucrose Phosphorylase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.
- Characteristics
- Synonyms: goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase
- Purification
- Anti-Sucrose phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Sucrose Phosphorylase [E.coli]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Application Note: Anti-Sucrose phosphorylase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Sucrose Phosphorylase
- Abstract
- Sucrose Phosphorylase Products
- Background
- Background: Sucrose phosphorylase catalyzes as Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate.
- Gene ID
- 4492194
- NCBI Accession
- YP_852475
- UniProt
- P76041
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