NEDD8 antibody

Catalog No. ABIN106594

Supplier Product No.: 200-401-427

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-427

Supplier: Rockland

Purpose: Rub1 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-Rub1 antibody, NEDD8-like protein RUB-1, Ubiquitin-like protein RUB 1

Purification: Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Rub1 Antibody is tested in western blot and defects a 6 kDa band corresponding to yeast Rub1.  Most yeast cell lysates can be used as a positive control without induction or stimulation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-NEDD8 antibody (ABIN106594)

Toro, Toth, Petroski: "The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics." in: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 14716-26, (2013) (PubMed).

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: RUB1 (NEDD8 Products)

Background: Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBL's) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBL's are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1.  Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDP's). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBL's, UDP's are not proteolytically processed or conjugated to other proteins.  Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters.  Nedd8 shows 80 % homology to ubiquitin.  The best known targets of Rub1 modification are members of the cullin family.  Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF).  The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein.  Rub1 modification may regulate SCF function or localization.

Gene ID: 851717

UniProt: Q03919

Pathways: Ubiquitin Proteasome Pathway