NEDD8 antibody

Catalog No. ABIN106594

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: Rub1 Antibody is deected as a 6 kDa band corresponding to yeast Rub1in immunoblotting.  Most yeast cell lysates can be used as a positive control without induction or stimulation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-NEDD8 antibody (ABIN106594)

Toro, Toth, Petroski: "The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics." in: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 14716-26, (2013) (PubMed).

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: Rub1 (NEDD8 Products)

Synonyms: NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody

Background: Ubiquitin-like proteins fall into  two classes: the first class, ubiquitin-like modifiers (UBL’s) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBL’s are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1.  Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDP’s). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBL’s, UDP’s are not proteolytically processed or conjugated to other proteins.  Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters.  Nedd8 shows 80% homology to ubiquitin.  The best known targets of Rub1 modification are members of the cullin family.  Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF).  The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein.  Rub1 modification may regulate SCF function or localization.
Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1

Gene ID: 851717

UniProt: Q03919

Pathways: Ubiquitin Proteasome Pathway