Rhodamine antibody

Catalog No. ABIN106554

Supplier Product No.: 200-301-246

Product Details

Target: Rhodamine

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Supplier Product No.: 200-301-246

Supplier: Rockland

Purpose: Rhodamine Antibody

Cross-Reactivity (Details): Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins.

Characteristics: Synonyms: Mouse Anti-Rhodamine Antibody

Purification: RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.

Immunogen Type: Other

Clone: 11H10

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:10,000

Application Note: This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Shimamura, Sasaki, Tanaka: "The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins." in: The Journal of biological chemistry, Vol. 288, Issue 2, pp. 1171-83, (2013) (PubMed).

Target Details

Target: Rhodamine

Target Type: Chemical

Background: Background: RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.