RFX5 antibody (AA 32-0)

Catalog No. ABIN106544

Product Details anti-RFX5 Antibody

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Binding Specificity: AA 32-0

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB), Gel Shift (GS)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG.  Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5.  This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-RFX5 antibody (ABIN106544)

Zhao, Xie, Liao, Zhang, Cao, Fei, Wang, Wei, Shao, Chen: "The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma." in: Oncology reports, Vol. 37, Issue 1, pp. 289-296, (2017) (PubMed).

Dille, Kleinschnitz, Kontchou, Nölke, Häcker: "In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport." in: Infection and immunity, Vol. 83, Issue 8, pp. 3268-80, (2015) (PubMed).

Xu, Sengupta, Seto, Smith: "Regulatory factor for X-box family proteins differentially interact with histone deacetylases to repress collagen alpha2(I) gene (COL1A2) expression." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9260-70, (2006) (PubMed).

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5 (RFX5 Products)

Synonyms: regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody

Background: Anti-RFX5 is specific for the RFX5 protien. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA.
Synonyms: DNA binding protein RFX5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X, 5 (influences HLA class II expression) antibody

Gene ID: 5993

UniProt: P48382