RPA2 antibody (AA 110-135)

Catalog No. ABIN106542

Supplier Product No.: 600-401-448

Product Details anti-RPA2 Antibody

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Binding Specificity: AA 110-135

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-448

Supplier: Rockland

Purpose: RFA2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against yeast RFA2.

Characteristics: Synonyms: rabbit anti-RFA2 antibody, RFA-2, RFA 2, Replication factor A protein 2, RF-A protein 2, Replication protein A 36 kDa subunit, DNA-binding protein BUF1, BUF-1, YNL312W, N0368

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2).   The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.  Yeast RFA2 is phosphorylated at the G₁/S transition and is dephosphorylated at mitosis.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody was tested for use in western blotting against RFA2 containing cell lysates and by ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:100 - 1:500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1000 - 1:3000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RFA2 (RPA2 Products)

Background: Background: RFA2 binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). It stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

Gene ID: 855404, 132475

UniProt: P26754

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA