Red Blood Cell antibody

Catalog No. ABIN106458

Supplier Product No.: 209-4139

Product Details anti-Red Blood Cell Antibody

Target: Red Blood Cell (RBC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Red Blood Cell antibody is un-conjugated

Application: Agglutination (AGG), Immunohistochemistry (IHC)

Supplier Product No.: 209-4139

Supplier: Rockland

Purpose: Human Red Blood Cell Antibody

Characteristics: Synonyms: rabbit Anti-human RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-human red blood cell, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocytes, anti-erythrocytes antibody

Purification: This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Human washed pooled Red Blood Cells (RBC)

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Human Red Blood Cells Antibody has been tested by agglutination and immunohistochemistry. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers. Specific conditions should be optimized by researcher.

Other: AGGLUTINATION TITER 1:32 - 1:64

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Red Blood Cell antibody (ABIN106458)

Arora, Hart, Manzella-Lapeira, Doritchamou, Narum, Thomas, Brzostowski, Rajagopalan, Doumbo, Traore, Miller, Pierce, Duffy, Crompton, Desai, Long: "NK cells inhibit Plasmodium falciparum growth in red blood cells via antibody-dependent cellular cytotoxicity." in: eLife, Vol. 7, (2019) (PubMed).

Target Details for Red Blood Cell

Target: Red Blood Cell (RBC)

Alternative Name: RBC (RBC Products)

Background: Background: Anti-Human Red Blood Cells Antibody recognizes human red blood cells and can be used in a variety of agglutination assays where agglutination or clumping of red blood cells is a positive indication of the presence of an analyte, virus particle or bacteria. Red blood cells (RBCs), also known as erythrocytes, are metabolically active cells that are highly adapted to serve their function in blood gas exchange (oxygen/CO2 transport). The red blood cells enable the transport of sufficient O2 between respiratory surfaces (lungs, gills) and metabolizing tissues by means of their high intracellular concentration of hemoglobin.